Quikchange primer design

A simple and efficient method for in vitro site-directed ...

The QuikChange™ method and its later modifications are popular used for ... Primer design is shown for each type of basic modification: site ...

First the mutagenic primers are annealed to denatured template DNA. (Note that all oligonucleotides are designed to bind the same strand of the template DNA.) ...

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Design primers for generating deletions, base substitutions, or additions using a flexible, accurate system.

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Primer design: the primers should have two or all of the following: 30-35 bases in length. mutation in the middle, or at least having 10 ...

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The QuikChange® Primer Design Program supports mutagenic primer design for your QuikChange mutagenesis experiments. Using primer design guidelines described ...

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The equation used for the melting temperature is: Tm = 81.5 + 0.41(%GC) - 675/N - % mismatch, where N = total number of bases. Paste your primer sequence below ...

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(2) Quickchange provides less significant amplification of the target DNA sequence than conventional PCR. Therefore, extra care should to be ...

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QuikChange Site Directed Mutagenesis. Primer Design Guidelines o Both of the mutagenic primers must contain the desired mutation and anneal to the same ...

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